Software as a Service
Smartfreez has specialized in modelling phase-change of complex biological (aqueous) systems for simulation of freezing and thawing related to the cryopreservation of biologics (such as proteins or cells). Smarfreez CFD models have been used to assist the design of Smartfreez methods and equipment, or its customer’s.

Smartfreez is currently providing Digital-Twins, to anticipate cryopreservation hurdles with minimal experimental testing, to reduce costs and shorten development period of freeze-sensitive molecules.

Instruments for Accelerated Cold-Stability Assessment
Smartfreez has designed systems that provide experimental assessment of degradation rates at low temperature.

The Cold Stability Accelerator measures isothermal degradation rates below freezing temperature without freezing the samples, enabling to discern the effect of temperature (by cold denaturation) from the interfacial stresses related to ice.

This system can also be used to anticipate in a short time frame (week) the long-term storage stability, which is relevant for optimization of formulation or cold chain logistics.

Instruments and Methods for Down-Scale Studies
Smartfreez has also developed the Micro-Scale-Down instrument, which can recreate the freeze-thaw stress of a bag or a bottle in a much smaller volume (as 5 mL). CFD simulation can generate the distribution of stresses during freezing and thawing. For example, the time that a sensitive molecule stays within the ice structure and above glass transition is directly related to its degradation rate during freeze-thaw.

This “stress-time” varies across the container; for example, the stress at the walls is different than at the center. The Micro-Scale-Down can convert a 5 mL vial into a representative physical model for freeze-thaw stress in 2L to 20 L manufacturing containers.

Integrative Freeze-Thaw Systems
SmartFreeZ systems use single-use technology to achieve high stability and consistency during freezing and thawing, either in bottles or bags, which can be made compatible with existing manufacturing platforms (as existing freezers).

Smartfreez Digital-Twins provide an accurate assessment of performance of Smartfreez systems, which can be used to maximize scalability, anticipate hurdles and accelerate time-to-market.