Our Expertise
Our team of specialized scientists is constantly working to stay on top of the cryopreservation sector.
We know the ins and outs of our products and we can confidently support you through your cryopreservation challenges!
Simulation of Freeze-Thaw Processes
Smartfreez is specialized in modelling phase-change of complex biological (aqueous) systems to simulate freezing and thawing related to the cryopreservation of biologics, such as proteins or cells.
We use our CFD models to aid in designing methods and equipment for our customers.
Additionally, we currently provide Digital-Twins to anticipate cryopreservation hurdles with minimal experimental testing, to reduce costs and shorted the development period of freeze-sensitive molecules.
Instruments for Accelerated Cold-Stability Assessment
We design systems that provide experimental assessments of degradation rates at low temperatures. The Cold Stability Accelerator measures isothermal degradation rates below freezing temperature, without freezing the samples.
This allows for discerning the effect of temperature, by cold denaturation, from the interfacial stresses related to ice.
Moreover, the system can anticipate long-term storage stability in a short time frame. This is relevant for optimising formulation or cold chain logistics.
Instruments and Methods for Down-Scale Studies
The Micro Scale-Down Instrument developed by Smartfreez can recreate the freeze-thaw stress of bottles or bags in a much smaller volume (below 10mL).
CFD simulation can account the time that a sensitive molecule stays within the ice structure and above glass transition. This is directly related to its degradation rate during freeze-thaw.
This “stress-time” varies across the container, for example, the stress at the walls is different than at the center. Based on CFD simulation, the Micro Sale-Down can convert a vial with less than 10 mL into a representative physical model for freeze-thaw stress in 2L to 20L manufacturing containers.
Integrative Freeze-Thaw Systems
We design single-use systems that achieve high stability and consistency during freezing and thawing of both bottles and bags. These systems can be easily made compatible with existing manufacturing platforms, such as existing freezers.
Furthermore, our Digital-Twins provide an accurate assessment of system performance, enabling customers to maximise scalability, anticipate hurdles, and accelerate time-to-market.